Course detail:
The course has been designed keeping in mind the need for much clearer and in-depth understanding of complete NGS concepts, chemistries, platforms and technologies, various workflows and applications. The whole workflow along with NGS terminologies with their meaning and significance are being discussed. After the course, one can expect to become champion of NGS concepts and confident to crack interviews or to undertake NGS lab operations. Learning NGS technology in depth with each and every concept is necessary to make career in the fast-growing genomics industry in clinical and non-clinical areas. The course will provide strong understanding of NGS and help in further career in the field.
* Once paid, course will be available for 15 days to complete
Curriculum
- 1 Section
- 14 Lessons
- 15 Days
- NEXT GENERATION SEQUENCING14
- 1.0Module 1: Introduction to Next Generation Sequencing (NGS)22 Minutes
- 1.1Module 2: Generations of seq, NGS Technologies 1- 454, SOLiD, Helicos PacBio27 Minutes
- 1.2Module 3: NGS Technologies 2- Illumina, Ion, MGI25 Minutes
- 1.3Module 4: NGS Technologies 3- Oxford Nanopore, 10x Genomics, Other new technologies16 Minutes
- 1.4Module 5: NGS Workflow 1- Overview, Sample QC, Fragmentation31 Minutes
- 1.5Module 6: NGS Workflow 2- End Repair, Adapters, Index, Size Selection, Library QC25 Minutes
- 1.6Module 7: NGS Workflow 3- Indexing, Amplicon lib, Transposon lib, Target Enrichment30 Minutes
- 1.7Module 8: Targeted Sequencing, Whole Genome, Exome, Panels, Metagenomic seq16 Minutes
- 1.8Module 9: NGS Analysis 1- Post-sequencing QC parameters part I21 Minutes
- 1.9Module 10: NGS Analysis 2- Post-sequencing QC parameters part II20 Minutes
- 1.10Module 11: NGS Analysis 3- Primary & Secondary Analysis, denovo assembly, Matepair library19 Minutes
- 1.11Module 12: NGS Analysis 4- Alignment, Mapping, Variant calling20 Minutes
- 1.12Module 13: NGS Analysis 5- Tertiary Analysis10 Minutes
- 1.13Module 14: Applications of NGS and Conclusion16 Minutes
Requirements
- Basic understanding of molecular biology
- Basic understanding of nucleic acids - DNA and RNA
- Understanding of basic laboratory equipment
Features
- Introduction to NGS and how it is different from conventional sequencing
- The concepts of massively parallel sequencing
- Various NGS platforms and their principles
- Detailed library preparation workflow and QC
- Concepts of Exome seq, WGS, Amplicon seq, Metagenomics etc
- Post sequencing QC and Primary, Secondary and Tertiary analysis
- Terminology used in NGS workflow and analysis
- Various applications of NGS

Please email the details
We require training NGS