Module 1: Introduction to PCR; Conventional PCR vs Real Time PCR

Module 2: Real Time PCR Chemistries 1, SYBR Green, Amplification curves

Module 3: Real Time PCR Chemistries 2. Probes based chemistries

Module 4: Applications part 1, Application overview, Detection

Module 5: Applications part 2, Absolute quant, std curve efficiency

Module 6: Applications part 3, Relative quant, Gene Expression

Module 8: Applications part 5, Methylation, miRNA, Multiplexing

Module 9: Applications part-6: Methylation analysis, miRNA, Multiplexing

Module 10: More fundamentals: Baseline correction, Passive ref dye ROX, Carry over contamination prevention by UDG

Module 11: Uses of the technology, Molecular Diagnostics and conclusion