Course detail:
Unlike learning only a specific application of Real Time PCR while working in a lab or watching videos, this course gives an ‘in-depth understanding’ of Real Time PCR technology and has been designed to cover everything – all important chemistries, all features and all applications – from basic to advance . The course is made for individuals with all levels of understanding including those who are already working in a lab using Real Time PCR for a specific use such as diagnostics. As the applications of the technology are vast and it is being used in several fields with different applications, people who are using it for a specific application need to understand all the concepts and various methods and chemistries for making themselves able to use in different ways. For academic individuals, faculty and students, it is very important to understand the technology in full in order to develop their skills to teach thoroughly or to answer all the questions in an interview they will be attending for jobs and admissions.
* Once paid, course will be available for 15 days to complete
Curriculum
- 1 Section
- 11 Lessons
- 15 Days
- Real Time PCR11
- 1.1Module 1: Introduction to PCR; Conventional PCR vs Real Time PCR18 Minutes
- 1.2Module 2: Real Time PCR Chemistries 1, SYBR Green, Amplification curves28 Minutes
- 1.3Module 3: Real Time PCR Chemistries 2. Probes based chemistries39 Minutes
- 1.4Module 4: Applications part 1, Application overview, Detection14 Minutes
- 1.5Module 5: Applications part 2, Absolute quant, std curve efficiency22 Minutes
- 1.6Module 6: Applications part 3, Relative quant, Gene Expression23 Minutes
- 1.7Module 7: Applications part 4, Variant detection, Endpoint genotyping, Tm analysis, Meltcurve genotyping, HRM32 Minutes
- 1.8Module 8: Applications part 5, Methylation, miRNA, Multiplexing23 Minutes
- 1.9Module 9: Applications part-6: Methylation analysis, miRNA, Multiplexing32 Minutes
- 1.10Module 10: More fundamentals: Baseline correction, Passive ref dye ROX, Carry over contamination prevention by UDG25 Minutes
- 1.11Module 11: Uses of the technology, Molecular Diagnostics and conclusion17 Minutes
Requirements
- Basic understanding of molecular biology
- Basic understanding of pipetting and measurements
- Basic understanding of conventional PCR (if not, you can attend the conventional PCR course)
- Basic knowledge of DNA and RNA
Features
- Introduction to the technology and how it is different from conventional PCR
- The concepts of Ct value and initial concentration
- Amplification curves and its features
- Various Real Time chemistries
- Various ways Real Time PCR can be used and applications
- Analysis concepts and methods
- Uses and special understandings
