Module 1: Introduction to Next Generation Sequencing (NGS)

Module 2: Generations of seq, NGS Technologies 1- 454, SOLiD, Helicos PacBio

Module 3: NGS Technologies 2- Illumina, Ion, MGI

Module 4: NGS Technologies 3- Oxford Nanopore, 10x Genomics, Other new technologies

Module 5: NGS Workflow 1- Overview, Sample QC, Fragmentation

Module 6: NGS Workflow 2- End Repair, Adapters, Index, Size Selection, Library QC

Module 7: NGS Workflow 3- Indexing, Amplicon lib, Transposon lib, Target Enrichment

Module 8: Targeted Sequencing, Whole Genome, Exome, Panels, Metagenomic seq

Module 9: NGS Analysis 1- Post-sequencing QC parameters part I

Module 11: NGS Analysis 3- Primary & Secondary Analysis, denovo assembly, Matepair library

Module 12: NGS Analysis 4- Alignment, Mapping, Variant calling

Module 13: NGS Analysis 5- Tertiary Analysis

Module 14: Applications of NGS and Conclusion