Live Online – Duration: 2 weeks (Dates are communicated in the advertisement or contact us)
With Mini project | Certificate | Actual datasets | industry-relevant workflows | End-to-end bioinformatics pipelines that employers and research labs truly value
“RNA-Seq in Bioinformatics: From Raw Reads to DEGs”
Modules Covered:
- Introduction to RNA-Seq and gene expression
- Quality check and trimming of raw reads (FASTQC, fastp)
- Read alignment to the reference genome
- Genome indexing using HISAT2
- Gene expression quantification across samples
- Differential gene expression analysis between conditions
- Visualization of gene expression results (DESeq2 etc)
- Biological interpretation of up/downregulated genes
- Mini project with full workflow and report submission
Learning Outcomes:
- Perform QC and trimming of RNA-Seq reads
- Align reads and generate count data
- Run differential expression analysis using DESeq2
- Visualize and interpret DEG results
- Present a mini project from end to end